烟草科技
2016-03-29 星期二
烟草农学:2020 ,7:1-9
刘跃东, 郑梅迎, 刘祥, 戴华伟, 王英俊, 彭玉龙, 刘明宏, 王毅, 孔凡玉, 张继光. 海泡石及生物炭对甲霜灵和镉复合污染条件下烟草生长发育和污染物含量的影响[J]. 烟草科技, 2020 (7): 1-9.
LIU Yuedong, ZHENG Meiying, LIU Xiang, DAI Huawei, WANG Yingjun, PENG Yulong, LIU Minghong, WANG Yi, KONG Fanyu, ZHANG Jiguang. Effects of sepiolite and biochar on growth and pollutant content in tobacco under combined metalaxyl and cadmium contamination[J]. Tobacco Science & Technology, 2020 (7): 1-9.

海泡石及生物炭对甲霜灵和镉复合污染条件下烟草生长发育和污染物含量的影响
刘跃东1, 郑梅迎1, 刘祥1, 戴华伟2, 王英俊2, 彭玉龙3, 刘明宏3, 王毅1, 孔凡玉1, 张继光1
1. 中国农业科学院烟草研究所, 山东省青岛市科苑经四路11号 266101;
2. 山东省烟草公司淄博市公司, 山东省淄博市张店区商场街143号 255000;
3. 贵州省烟草公司遵义市公司, 贵州省遵义市汇川区延安路143号 563000
摘要:
为明确不同土壤改良剂(海泡石和生物炭)对植烟土壤甲霜灵与镉复合污染条件下烟草生长发育特征与污染物含量的影响,采用盆栽试验研究了甲霜灵与镉复合污染条件下,添加海泡石和生物炭对烤烟植物学性状、不同生育期烤烟不同部位生物量、根系发育及污染物吸收的影响。结果表明:①添加量20 g·kg-1海泡石或生物炭均能不同程度地促进烟株生长和生物量的积累,与对照相比海泡石和生物炭两者等量(10 g·kg-1)配施,可显著增加烟株株高、叶片数、根生物量、根冠比、主根长和根体积等(P<0.05)。②海泡石与生物炭及其配施处理均能降低烟叶和根部的甲霜灵含量并缩短甲霜灵在烟叶中的半衰期,但处理间差异不显著。③与对照相比,3种改良剂处理中烟草根部及叶部对镉元素的吸收高峰均延迟至烟株现蕾期,且在根和叶中镉的富集系数降低。因此,海泡石与生物炭等量配施既可显著促进烟株的生长发育,又可缓解镉与甲霜灵对烟株生长的毒害作用,是甲霜灵和镉复合污染条件下烟田改良的较有效措施。
关键词:    烟草    改良剂    海泡石    生物炭    复合污染    甲霜灵        根系    生长发育   
Effects of sepiolite and biochar on growth and pollutant content in tobacco under combined metalaxyl and cadmium contamination
LIU Yuedong1, ZHENG Meiying1, LIU Xiang1, DAI Huawei2, WANG Yingjun2, PENG Yulong3, LIU Minghong3, WANG Yi1, KONG Fanyu1, ZHANG Jiguang1
1. Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong, China;
2. Zibo Branch of Shandong Provincial Tobacco Company, Zibo 255000, Shandong, China;
3. Zunyi Branch of Guizhou Provincial Tobacco Company, Zunyi 563000, Guizhou, China
Abstract:
In order to clarify the influences of different soil amendments on the growth and development of tobacco and the contents of pollutants in tobacco, the effects of applying sepiolite and biochar on botanical traits of flue-cured tobacco, biomasses of different parts of tobacco at different growing stages, root development and pollutant absorption under the combined pollution of metalaxyl and cadmium were studied via pot experiments. The results showed that adding sepiolite or biochar at the rate of 20 g·kg-1 promoted the growth of tobacco and biomass accumulation to different extents. Compared with the control, the equal application of sepiolite and biochar(at the rate of 10 g·kg-1 each)significantly(P<0.05) increased the height, leaf number, root biomass, root top ratio, main root length and root volume of tobacco plant. Applying sepiolite, biochar or their combination decreased the metalaxyl content in leaves and roots, and shortened the half-life of metalaxyl in leaves, however there were no significant differences among the different treatments. In addition, the three soil amendments delayed the cadmium absorption peak of tobacco roots and leaves until the flower-bud appearing stage and decreased the cadmium enrichment coefficients in tobacco roots and leaves. Therefore, the equal application of sepiolite and biochar is an effective means for improving the soil equality in tobacco fields since it can significantly promote tobacco growth and development by reducing the harms of cadmium and metalaxyl to tobacco plants.
Key words:    Tobacco    Amendment    Sepiolite    Biochar    Combined pollution    Metalaxyl    Cadmium    Root    Growth and development   
收稿日期: 2019-12-15     修回日期: 2020-04-03
DOI: 10.16135/j.issn1002-0861.2019.0534
基金项目: 中国农业科学院科技创新工程(ASTIP-TRIC06);山东省烟草公司科技项目“淄博烟区中微量元素特征及其调控技术研究”(201707);贵州遵义市烟草公司科技项目“遵义烟田土壤酸化阻控关键技术研究与应用”(201703)。
通讯作者: 张继光,E-mail:zhangjiguang@caas.ac.cn     Email:zhangjiguang@caas.ac.cn
作者简介: 刘跃东(1992-),在读博士,研究方向:烟田生态与污染修复技术研究。E-mail:liuyuedongxc@163.com
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参考文献:
[1] 全国土壤污染状况调查公报[EB/OL]. (2014-04-17). http://www.zhb.gov.cn/gkml/hbb/qt/201404/t20140417_270670.htm. Report on the national general survey of soil contamination[EB/OL]. (2014-04-17).http://www.zhb.gov.cn/gkml/hbb/qt/201404/t20140417_270670.htm
[2] Wang P, Zhou D M, Kinraide T B, et al. Cell membrane surface potential(ψ0)plays a dominant role in the phytotoxicity of copper and arsenate[J]. Plant Physiology,2008,148(4):2134-2143.
[3] 石贵玉. 重金属Cr6+对水稻幼苗的毒害效应[J]. 广西科学,2004,11(2):154-156. SHI Guiyu. The Toxic effect of heavy metal Cr6+ on rice seedling[J]. Guangxi Sciences, 2004,11(2):154-156.
[4] 曾德武,李帆,赵建武,等. 烟草重金属污染及其防控措施研究进展[J]. 中国烟草科学,2014,35(1):127-132. ZENG Dewu,LI Fan,ZHAO Jianwu,et al. Advance in tobacco heavy metal pollution and its controlling measures[J]. Chinese Tobacco Science, 2014, 35(1):127-132.
[5] 张凯,谢利丽,武云杰,等. 烟草黑胫病的发生及综合防治研究进展[J]. 中国农业科技导报,2015,17(4):62-70. ZHANG Kai,XIE Lili,WU Yunjie,et al. Research progress on occurrence of tobacco black shank and its integrated control[J]. Journal of Agricultural Science and Technology,2015,17(4):62-70.
[6] Xie Y H,Zhang Y G,Zhu L Q,et al. Progress on integrated control of tobacco black shank[J]. Agricultural Biotechnology,2015,4(4):37-41.
[7] Wilson P C,Whitwell T,Klaine S J. Metalaxyl toxicity, uptake, and distribution in several ornamental plant species[J]. Journal of Environmental Quality,2001,30(2):411-417.
[8] 王晓岚,钱娇娟,刘沁雨,等. 农药-重金属铅复合污染生态毒理效应研究进展[J]. 安徽农业科学, 2018,46(6):21-25. WANG Xiaolan,QIAN Jiaojuan,LIU Qinyu,et al. Research progress on ecotoxicological effects of compound pollution of pesticides and heavy metals[J]. Journal of Anhui Agricultural Sciences,2018,46(6):21-25.
[9] Wang J H,Ding H,Lu Y T,et al. Combined effects of cadmium and butachlor on microbial activities and community DNA in a paddy soil[J]. Pedosphere, 2009,19(5):623-630.
[10] 孙约兵,徐应明,史新,等. 海泡石对镉污染红壤的钝化修复效应研究[J]. 环境科学学报,2012,32(6):1465-1472. SUN Yuebing, XU Yingming, SHI Xin, et al. The effects of sepiolite on immobilization remediation of Cd contaminated red soil[J]. Acta Scientiae Circumstantiae, 2012, 32(6):1465-1472.
[11] Chen H M, Zheng C R, Tu C, et al. Chemical methods and phytoremediation of soil contaminated with heavy metals[J]. Chemosphere,2000,41(1/2):229-234.
[12] Walker D J, Clemente R, Bernal M P. Contrasting effects of manure and compost on soil pH, heavy metal availability and growth of Chenopodium album L. in a soil contaminated by pyritic mine waste[J]. Chemosphere,2004,57(3):215-224.
[13] 任露陆, 吴文成,宋清梅,等. 不同类型固化材料对Cu污染水稻土的修复效果差异[J]. 环境化学, 2016,35(3):548-554. REN Lulu,WU Wencheng, SONG Qingmei, et al. Effects of four types immobilizers on Cu immobilization in contaminated paddy soil[J]. Environmental Chemistry,2016, 35(3):548-554.
[14] 尤方芳,赵铭钦,孙翠红,等. 生物炭与不同肥料配施对镉胁迫下烟叶和土壤中镉含量的影响[J]. 中国农业科技导报,2016,18(4):115-123. YOU Fangfang,ZHAO Mingqin,SUN Cuihong,et al. Effects of combined application of biochar and different fertilizers on Cadmium content in tobacco and soil under Cadmium stress[J]. Journal of Agricultural Science and Technology,2016,18(4):115-123.
[15] 李亮,张翔,毛家伟,等. 生物有机肥在烟草上的应用研究进展及展望[J]. 中国农学通报,2016,32(18):47-52. LI Liang,ZHANG Xiang,MAO Jiawei,et al. Advances in bio-organic fertilizer application to tobacco[J]. Chinese Agricultural Science Bulletin,2016,32(18):47-52.
[16] 朱先强,石林. 矿物质钝化剂对铅铜复合型污染土壤的修复作用[J]. 生态环境学报,2017,26(4):708-713. ZHU Xianqiang, SHI Lin. Remediation effects of mineral amendment on Pb and Cu contaminated soil[J]. Ecology and Environmental Sciences,2017, 26(4):708-713.
[17] Ding Y,Liu Y G,Liu S B,et al. Biochar to improve soil fertility. A review[J]. Agronomy for Sustainable Development,2016,36(2):36.
[18] 孟莉蓉,俞浩丹,杨婷婷,等. 2种生物炭对Pb、Cd污染土壤的修复效果[J]. 江苏农业学报,2018,34(4):835-841. MENG Lirong,YU Haodan,YANG Tingting,et al. Immobilization of two biochars to Pb, Cd in contaminated soils[J]. Jiangsu Journal of Agricultural Sciences,2018,34(4):835-841.
[19] 陈立,王丹,龙婵,等. 三种螯合剂对芥菜修复铀镉复合污染土壤的影响[J]. 农业环境科学学报, 2018,37(8):1690-1697. CHEN Li,WANG Dan,LONG Chan,et al. Effects of three kinds of chelating agents on U and Cd phytoremediation in Brassica juncea L.[J]. Journal of Agro-Environment Science,2018,37(8):1690-1697.
[20] 鲁如坤. 土壤农业化学分析方法[M]. 北京:中国农业科技出版社,1999. LU Rukun. The analysis method of soil agricultural chemistry[M]. Beijing:China Agriculture Scientech Press,1999.
[21] 刘国顺. 烟草栽培学[M]. 北京:中国农业出版社, 2003. LIU Guoshun. Tobacco cultivation[M]. Beijing:China Agricultural Press,2003.
[22] 侯冬岩,回瑞华,李铁纯,等. 电感耦合等离子体质谱法(ICP-MS)同时测定正山小种红茶中18种微量元素[J]. 中国无机分析化学,2012,2(2):52-56. HOU Dongyan, HUI Ruihua, LI Tiechun, et al. Simultaneous determination of 18 trace elements in lapsangsouchong black tea by inductively coupled plasma mass spectrometry (ICP-MS)[J]. Chinese Journal of Inorganic Analytical Chemistry, 2012, 2(2):52-56.
[23] Buscaroli A. An overview of indexes to evaluate terrestrial plants for phytoremediation purposes(review)[J]. Ecological Indicators, 2017, 82:367-380.
[24] 龙毅,孟凡栋,王常顺,等. 高寒草甸主要植物地上地下生物量分布及退化对根冠比和根系表面积的影响[J]. 广西植物,2015,35(4):532-538. LONG Yi,MENG Fandong,WANG Changshun,et al. Above-and below-ground biomass distribution of main alpine meadow plants and impact of degradation on root/shoot ratio and root area[J]. Guihaia,2015,35(4):532-538.
[25] 杜彩艳,段宗颜,曾民,等. 田间条件下不同组配钝化剂对玉米(Zea mays)吸收Cd、As和Pb影响研究[J]. 生态环境学报,2015,24(10):1731-1738. DU Caiyan,DUAN Zongyan,ZENG Min,et al. Effects of different combined amendments on cadmium,arsenic and lead absorption of maize under fieldconditions[J]. Ecology and Environmental Sciences,2015,24(10):1731-1738.
[26] 彭丽成,黄占斌,石宇,等. 环境材料对Pb、Cd污染土壤玉米生长及土壤改良效果的影响[J]. 中国生态农业学报,2011,19(6):1386-1392. PENG Licheng, HUANG Zhanbin, SHI Yu, et al. Effects of environmental materials on maize growth and soil remediation of Pb and Cd contaminated soils[J]. Chinese Journal of Eco-Agriculture, 2011,19(6):1386-1392.
[27] 许跃奇,赵铭钦,尤方芳,等. 生物炭与常规施肥对烟草生长及镉污染吸收的影响[J]. 土壤,2016,48(3):510-515. XU Yueqi, ZHAO Mingqin,YOU Fangfang, et al. Effects of biochar and conventional fertilization on tobacco growth and Cadmium uptake[J]. Soils,2016, 48(3):510-515.
[28] 李丽君,刘平,白光洁,等. 海泡石改良土壤效果研究[J]. 水土保持学报,2012,26(2):275-278,283. LI Lijun,LIU Ping,BAI Guangjie,et al. Research on the amelioration effect of meerschaum in soil[J]. Journal of Soil and Water Conservation, 2012, 26(2):275-278,283.
[29] Sun Y B, Sun G H, Xu Y M, et al. In situ stabilization remediation of cadmium contaminated soils of wastewater irrigation region using sepiolite[J]. Journal of Environmental Sciences, 2012,24(10):1799-1805.
[30] 郑雄志,李宏光,龙世平,等. 几种解毒剂对烟田二氯喹啉酸次生药害的修复效果[J]. 南方农业学报, 2013,44(3):426-430. ZHENG Xiongzhi,LI Hongguang,LONG Shiping,et al. Remediation effects of different antidotes on phytotoxicity of quincloracresidue in tobacco soil[J]. Journal of Southern Agriculture, 2013, 44(3):426-430.
[31] Yu X Y,Ying G G,Kookana R S. Reduced plant uptake of pesticides with biochar additions to soil[J]. Chemosphere,2009,76(5):665-671.
[32] 孙朋成,黄占斌,刘陆涵. 石棉尾矿渣在土壤重金属及农药污染治理中的应用[J]. 环境工程,2015,33(7):144-147. SUN Pengcheng,HUANG Zhanbin,LIU Luhan. The application of asbestos tailing residue in heavy metal and pesticide pollution control in soil[J]. Environmental Engineering,2015,33(7):144-147.
[33] 徐应明,梁学峰,孙国红,等. 海泡石表面化学特性及其对重金属Pb2+ Cd2+和Cu2+吸附机理研究[J]. 农业环境科学学报, 2009, 28(10):2057-2063. XU Yingming,LIANG Xuefeng,SUN Guohong,et al. Surface chemical characteristics of sepiolites and their adsorption mechanisms of Pb2+,Cd2+ and Cu2+[J]. Journal of Agro-Environment Science,2009,28(10):2057-2063.
[34] 李明德,童潜明,汤海涛,等. 海泡石对镉污染土壤改良效果的研究[J]. 土壤肥料,2005(1):42-44. LI Mingde,TONG Qianming,TANG Haitao,et al. Study on the amelioration effect of meerschaum in Cd contaminated soil[J]. Soils and Fertilizers,2005(1):42-44.
[35] 孙约兵,王朋超,徐应明,等. 海泡石对镉-铅复合污染钝化修复效应及其土壤环境质量影响研究[J]. 环境科学,2014,35(12):4720-4726. SUN Yuebing,WANG Pengchao,XU Yingming,et al. Immobilization remediation of Cd and Pb contaminated soil:remediation potential and soil environmental quality[J]. Environmental Science, 2014, 35(12):4720-4726.
[36] 张丽,侯萌瑶,安毅,等. 生物炭对水稻根际微域土壤Cd生物有效性及水稻Cd含量的影响[J]. 农业环境科学学报,2017,36(4):665-671. ZHANG Li,HOU Mengyao,AN Yi,et al. Effects of biochar on Cd bioavailability in rhizosphere microenvironment of cadmium-polluted paddy and Cd content in rice[J]. Journal of Agro-Environment Science, 2017, 36(4):665-671.
[37] 孙慧,吴迪,王宇,等. 海泡石与生物炭对镉污染红壤的钝化修复研究[J]. 江苏农业科学,2018,46(14):289-291. SUN Hui, WU Di, WANG Yu, et al. Study on passivation repair of cadmium-contaminated red soil by sepiolite and biochar[J]. Jiangsu Agricultural Sciences, 2018,46(14):289-291.
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