烟草科技
2016-03-29 星期二
烟草化学:2020 ,9:25-32
程皖燕, 王红娟, 陈欢, 付亚宁, 王安, 刘勇, 侯宏卫, 胡清源. 烟碱偏好模型不同阶段大鼠海马组织中23种细胞因子水平的变化[J]. 烟草科技, 2020 (9): 25-32.
CHENG Wanyan, WANG Hongjuan, CHEN Huan, FU Yaning, WANG An, LIU Yong, HOU Hongwei, HU Qingyuan. Variations of 23 cytokine levels in rat hippocampus at different stages of a nicotine preference model[J]. Tobacco Science & Technology, 2020 (9): 25-32.

烟碱偏好模型不同阶段大鼠海马组织中23种细胞因子水平的变化
程皖燕1,2,3, 王红娟2, 陈欢2, 付亚宁2, 王安3, 刘勇3, 侯宏卫2, 胡清源2
1. 安徽大学物质科学与信息技术研究院, 合肥市经济开发区九龙路111号 230601;
2. 国家烟草质量监督检验中心, 郑州高新技术产业开发区翠竹街6号 450001;
3. 中国科学院合肥物质科学研究院, 合肥市蜀山湖路350号 230031
摘要:
为了探究烟碱对大鼠海马组织中炎症相关细胞因子表达的影响,利用条件位置偏好(Conditioned place preference,CPP)装置,分别以0.2和0.6 mg/kg烟碱剂量成功构建大鼠烟碱CPP获得、消退和重建模型,应用悬液芯片技术对大鼠海马组织中23种炎症相关的细胞因子水平进行同时检测。通过单因素方差分析探讨了烟碱偏好不同阶段大鼠海马组织中表达水平有显著差异的细胞因子。结果表明:①低和较高剂量下,烟碱能够促进细胞因子白细胞介素(Interleukin,IL)-1α和IL-10的表达,抑制IL-6、IL-18、生长相关癌基因/角细胞趋化因子(Growth-related oncogene/keratinocyte chemoattractant,GRO/KC)以及受激活调节正常T细胞表达和分泌因子(Regulated upon activation normal T cell expressed and secreted factor,RANTES)的表达;②大鼠海马中GRO/KC水平易受烟碱影响,单一烟碱作用会降低GRO/KC的表达,在获得-消退阶段,该降低作用减弱,在消退-重建阶段该降低作用再次恢复至获得阶段,该细胞因子可能参与烟碱的潜在抗炎作用机制;③其他5种细胞因子表达量的变化趋势与GRO/KC不相同甚至相反,有必要在接下来的研究中进一步通过动物炎症模型探究烟碱对炎症影响的作用机制。
关键词:    烟碱    偏好模型    海马组织    悬液芯片技术    细胞因子    炎症   
Variations of 23 cytokine levels in rat hippocampus at different stages of a nicotine preference model
CHENG Wanyan1,2,3, WANG Hongjuan2, CHEN Huan2, FU Yaning2, WANG An3, LIU Yong3, HOU Hongwei2, HU Qingyuan2
1. Institute of Physical Science and Information Technology, Anhui University, Hefei 230601, China;
2. National Tobacco Quality Supervision&Test Center, Zhengzhou 450001, China;
3. Hefei Institute of Physical Sciences, Chinese Academy of Sciences, Hefei 230031, China
Abstract:
To investigate the effects of nicotine on the expression of inflammation-related cytokines in the hippocampus of rats, a conditional place preference (CPP) device was used to establish the acquisition, extinction and reinstatement of nicotine CPP in rats at the nicotine doses of 0.2 and 0.6 mg/kg. A total of 23 inflammation-related cytokines were detected simultaneously in the hippocampus at different stages (acquisition, extinction and reinstatement) by the suspension chip technique. The one-way variance analysis was adopted to analyze the differential cytokine expressions in the hippocampus at different stages using a nicotine preference model. The results showed that:1) Nicotine promoted the expressions of interleukin (IL)-1α and IL-10 and inhibited the expressions of IL-6, IL-18, growth-related oncogene/keratinocyte chemoattractant (GRO/KC) and regulated upon activation normal T cell expressed and secreted factor (RANTES) at low and higher doses. 2) The GRO/KC level in rat hippocampus was susceptible to nicotine, which reduced GRO/KC expression, while the reduction effect was weakened during the acquisition-extinction stage and was restored to the level at the acquisition stage during the extinction-reinstatement stage, the evidence therefore suggested that GRO/KC might be involved in the potential anti-inflammatory mechanism of nicotine. 3) The variation trends of the other five cytokine levels were different or even contrary to GRO/KC. Further studies are needed to understand the inflammatory mechanism of nicotine through the rat and other animal inflammation models.
Key words:    Nicotine    Preference model    Hippocampus    Suspension chip technology    Cytokine    Inflammation   
收稿日期: 2020-01-20     修回日期: 2020-05-10
DOI: 10.16135/j.issn1002-0861.2020.0039
基金项目: 国家烟草专卖局重点项目“烟碱致瘾性阈值研究”(110201402037)和重大专项项目“基于实时递送评价的口含烟烟碱释放规律研究及应用”[110201901005(XX-05)]。
通讯作者: 胡清源,E-mail:huqy1965@163.com     Email:huqy1965@163.com
作者简介: 程皖燕(1995-),在读硕士研究生,研究方向:烟碱偏好。E-mail:1179205486@qq.com
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