烟草科技
2016-03-29 星期二
烟草农学:2020 ,10:1-9
向立刚, 汪汉成, 郭华, 李芝义, 郑苹, 蔡刘体, 余知和. 基于盆栽试验的苯并噻唑硫酮对感染青枯病烟株根围土壤细菌群落结构的影响[J]. 烟草科技, 2020 (10): 1-9.
XIANG Ligang, WANG Hancheng, GUO Hua, LI Zhiyi, ZHENG Ping, CAI Liuti, YU Zhihe. Effects of 3-methylbenzothiazole-2-thione on community structure of bacteria in rhizospheric soil of tobacco plants infected by bacterial wilt[J]. Tobacco Science & Technology, 2020 (10): 1-9.

基于盆栽试验的苯并噻唑硫酮对感染青枯病烟株根围土壤细菌群落结构的影响
向立刚1,2, 汪汉成1, 郭华3, 李芝义4, 郑苹1, 蔡刘体1, 余知和2
1. 贵州省烟草科学研究院, 贵阳市龙滩坝路29号 550081;
2. 长江大学生命科学学院, 湖北省荆州市荆秘路88号 434025;
3. 贵州省疾病预防控制中心, 贵阳市八鸽岩路101号 550004;
4. 荆州市荆州区马山镇农业技术服务中心, 湖北省荆州市荆州区马山镇马南路28号 434031
摘要:
为明确玉米根系分泌物3-甲基-2(3H)苯并噻唑硫酮(简称苯并噻唑硫酮)灌根处理对感染青枯病烟株根围土壤细菌群落的影响机制,通过盆栽试验,采用Illumina MiSeq高通量测序技术对苯并噻唑硫酮灌根处理当天(0 d),处理后2、7、20和40 d共5个时期烟株根围土壤中细菌群落结构进行分析。结果表明:①苯并噻唑硫酮灌根40 d后烟株无明显青枯病症状,而对照灭菌蒸馏水灌根40 d后烟株均枯萎死亡。②苯并噻唑硫酮灌根处理后,烟株根围细菌Alpha多样性指数中Sobs指数、Chao1指数以及香农指数(Shannon)升高。③苯并噻唑硫酮处理后5个时期的共有优势菌门为放线菌门(Actinobacteria)、变形菌门(Proteobacteria)和绿弯菌门(Chloroflexi);共有优势菌属为Pseudarthrobacter、鞘氨醇单胞菌属(Sphingomonas)和芽单胞菌属(Gemmatimonas)。④苯并噻唑硫酮灌根处理可提高烟株根围土壤中放线菌门相对丰度,降低Saccharibacteria菌门相对丰度,增加类诺卡氏属(Nocardioides)、Streptomyces和链霉菌属(Streptomyces)相对丰度,降低鞘氨醇单胞菌属(Sphingomonas)相对丰度。因此,苯并噻唑硫酮灌根能有效防治烟草青枯病,并影响烤烟根围细菌群落结构,增加有益菌相对丰度。
关键词:    烟草青枯病    细菌群落    多样性    Illumina-MiSeq高通量测序    青枯雷尔氏菌    苯并噻唑硫酮   
Effects of 3-methylbenzothiazole-2-thione on community structure of bacteria in rhizospheric soil of tobacco plants infected by bacterial wilt
XIANG Ligang1,2, WANG Hancheng1, GUO Hua3, LI Zhiyi4, ZHENG Ping1, CAI Liuti1, YU Zhihe2
1. Guizhou Academy of Tobacco Science, Guiyang 550081, China;
2. College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China;
3. Guizhou Center for Disease Control and Prevention, Guiyang 550004, China;
4. Mashan Town Agricultural Technology Service Center of Jingzhou District of Jingzhou City, Jingzhou 434031, Hubei, China
Abstract:
In order to clarify the affecting mechanism of root irrigation with 3-methylbenzothiazole-2-thione secreted by corn roots on the community structure of bacteria in the rhizospheric soil of tobacco plants infected by bacterial wilt, Illumina MiSeq high-throughput sequencing technology was used to analyze the community structure of bacteria in the rhizospheric soil of tobacco plants infected by bacterial wilt on the day of the root irrigation as well as the 2nd, 7th, 20th and 40th days after root irrigation through pot experiments. The results showed that:1) There was no obvious bacterial wilt symptom 40 days after root irrigation with 3-methylbenzothiazole-2-thione, while all tobacco plants withered and died 40 days after root irrigation with sterilized distilled water. 2) After root irrigation with 3-methylbenzothiazole-2-thione, Sobs index, Chao1 index and Shannon index of Alpha diversity indexes of bacteria in rhizospheric soil of tobacco plants increased. 3) The dominant phyla were Actinobacteria, Proteobacteria and Chloroflexi and the dominant genera were Pseudarthrobacter, Sphingomonas and Gemmatimonas in all the five stages after irrigation with 3-methylbenzothiazole-2-thione. 4) Root irrigation with 3-methylbenzothiazole-2-thione increased the relative abundances of Actinobacteria, Nocardioides and Streptomyces and decreased the relative abundances of Saccharacteria and Sphingomonas in the rhizospheric soil of tobacco plants. Therefore root irrigation with 3-methylbenzothiazole-2-thione can effectively control tobacco bacterial wilt, affect the community structure of bacteria in the rhizospheric soil of flue-cured tobacco, and increase the relative abundances of beneficial bacteria.
Key words:    Tobacco bacterial wilt    Bacterial community    Diversity    Illumina MiSeq high-throughput sequencing    Ralstonia solanacearum    3-Methylbenzothiazole-2-thione   
收稿日期: 2019-11-11     修回日期: 2020-04-18
DOI: 10.16135/j.issn1002-0861.2019.0482
基金项目: 国家烟草专卖局科技项目“烟草主要叶斑类病害监测预警及绿色防控技术研究与应用”[110202001035(LS-04)];贵州省科技厅优秀青年人才培养计划项目“集约化育苗下烟草立枯病的流行规律及其防控技术研究”(黔科合平台人才[2017]5619);中国烟草总公司贵州省公司科技项目“‘互联网+烟草植保’的研究与应用”(201714)、烟草青枯菌与烟草疫霉协同侵染烟草的微生态研究(201914)。
通讯作者: 汪汉成,E-mail:xiaobaiyang126@hotmail.com;余知和,E-mail:zhiheyu@hotmail.com     Email:xiaobaiyang126@hotmail.com;zhiheyu@hotmail.com
作者简介: 向立刚(1994-),在读硕士研究生,研究方向:植物保护。E-mail:1475206901@qq.com
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