试验土壤中烟草青枯病菌的RT-qPCR检测分析

RT-qPCR detection and quantitative analysis of Ralstonia solanacearum in soil

摘要: 为有效防治烟草青枯病，利用实时荧光定量PCR（Real-time quantity PCR，RT-qPCR）技术，对土壤中烟草青枯病菌进行了定量检测，并建立了土壤中烟草青枯病菌的RT-qPCR检测方法。结果表明，该方法对试验土壤青枯菌的最低检测浓度为5×10² cfu/g土壤，RT-qPCR检测技术对土壤中烟草青枯病菌的灵敏度比常规PCR检测技术提高了10倍。

Abstract: To effectively control tobacco bacterial wilt, real-time quantitative PCR (RT-qPCR) technique was developed and used to quantitatively detect Ralstonia solanacearum in soil. The results showed that the lowest concentration of Ralstonia solanacearum in soil detected by the developed system was 5×10² cfu/g. Comparing with conventional PCR detection, the sensitivity of RT-qPCR detection to Ralstonia solanacearum in soil increased by 10 times.