Abstract: In order to analyze the tralomethrin and deltamethrin in tobacco simultaneously, the extract was preliminarily purified after tobacco sample was extracted by QuRChERS pretreatment, the extract was further purified with Florisil solid phase extraction column. Tralomethrin and deltamethrin were determined by ultra performance convergence chromatography (UPC2) with non-polar HSS C₁₈ SB column as solid phase and methanol as mobile phase auxiliary agent. The results showed that it only took 6.5 minutes to complete the analysis of tralomethrin and deltamethrin residues, the linear range of this method were 0.5-25.0 mg/kg with the correlation coefficient (R²) more than 0.999 5 and relative standard deviation less than 10%. The average standard addition recoveries and detection limits of tralomethrin and deltamethrin were 92.6%, 84.3% and 0.33, 0.23 mg/kg, respectively. This method advantages over gas chromatography, the later fails to detect tralomethrin.