Abstract: In order to reduce the content of starch in flue-cured tobacco leaves, three curing means were tested and compared with current curing means(CK), including low temperature high humidity longer period flue-curing(W), cellulose-aid flue-curing(M) and high temperature-induced amylase pre-activating flue-curing(BK), separately. The starch content in tobacco leaf was determined by iodine colorimetry, and the activity of amylase by 3, 5-dinitrosalicylic acid(DNS) colorimetry. The results showed that: 1) The starch contents in tobacco leaves cured by CK, W, M, BK were 6.54%, 6.06%, 5.08%, 3.13%, respectively. 2) The amylase activity in fresh tobacco leaf was 0.25 U, increased to 0.31 U after curing for 3 hours by CK. For BK, the amylase activity in tobacco leaf pre-activated by high temperature reached 0.49 U, the amylase activity during curing process exhibited a profile with two peaks. High temperature-induced amylase pre-activating flue-curing means reduced the starch content in tobacco leaf more obviously.