Abstract: In order to shorten the period of seedling culturing and improve the quality of tobacco seedlings,a contrast experiment was conducted by applying seedling fertilizers at different rates into seedling culturing substrates.The results showed that comparing with the control(CK),the period of seedling culturing reduced by 8 days when seedling fertilizers were applied into the substrates at a rate of 520 g/m³.All of seedling growing stages were shortened,particularly the duration from seedling germination to little cross stage decreased by 3.38 days.The number and weight of roots,the agriculture traits,root vitality and the chlorophyll content and nitrate reductase activity of leaves reached to their highest.Therefore,applying appropriate amount(520 g/m³) seedling fertilizers into substrates can promote the growth and development of tobacco seedlings,obviously shorten seedling growing period,and the cultured seedlings possess vigorous and well developed root systems.