Abstract: In order to have a sound grasp of the quality characteristics of flue-cured tobacco in Yuxi and provide data support for tobacco planting,tobacco blending and cigarette manufacturing,the main chemical components in 25 430 samples of 5 cultivars(K326,KRK26,NC297,NC71 and Hongda) collected from 9 areas in Yuxi,Yunnan from 2009 to 2012 were determined,and the variance analysis of chemical and harmonious indexes was performed.The appearance quality and sensory quality of samples of the 5 cultivars from 2012 were evaluated.The results showed that:1) The contents of nicotine,total sugar,chlorine and potassium of flue-cured tobacco from Yuxi were basically in the appropriate ranges required by Hongta Tobacco(Group) Corporation,however the difference between total sugar and reducing sugar and the ratio of non-nicotine N to total N were not all up to the required targets.2) The cultivars were different in chemistry and harmony,K326 was higher in chlorine content and lower in potassium content;KRK26 presented higher sugar,chlorine,potassium contents,lower total nitrogen content,smaller ratios of total nitrogen/nicotine,potassium/chlorine,non-nicotine nitrogen/total nitrogen,and greater difference between total sugar and reducing sugar;NC297 presented lower chlorine content,smaller ratios of total nitrogen/nicotine and non-nicotine nitrogen/total nitrogen,larger difference between total sugar and reducing sugar;NC71 possessed higher nicotine and nitrogen contents,lower sugar content,smaller ratio of sugar/nicotine and difference between total sugar and reducing sugar;Hongda possessed lower nicotine and chlorine contents,higher reducing sugar content,larger ratios of sugar/nicotine,total nitrogen/nicotine,non-nicotine nitrogen/total nitrogen,and smaller difference between total sugar and reducing sugar.3) The appearance quality of the 5 cultivars was good in general,though there existed some differences between cultivars.The comprehensive sensory quality of the 5 cultivars was good,each cultivar held special character of its own and satisfied the requirements of Hongta Group for tobacco leaves.