Abstract: Long running time,high energy consumption and high running cost are the problems associated with the air conditioning system with primary recycling air in cigarette enterprises running under coordinate regulation mode in summer.Therefore a temperature-humidity separate control method for air conditioning system based on single cold source was developed by taking cut tobacco storing room as an example,meantime,the control units of the air conditioning system was improved as following:1) Installing another surface cooler for dehumidification control and the original surface cooler was used as the secondary cooler for cooling control.The two surface coolers were connected in series,the input cold water of the secondary cooler come from the first cooler with 3-5℃ temperature rise to implement the separate temperature-humidity control by single cold source.2) Adding an independent fresh air dehumidifying unit in the fresh air section to change the control mode from temperature and humidity coordinate regulation to separate control in summer.The results showed that the separate temperature-humidity control method effectively avoided the cold-heat offset and energy waste in temperature and humidity coordinate regulation mode in summer,the energy consumption of air conditioning unit in dehumidification decreased by 25.5%,and the running efficiency of air conditioning system was promoted.