黑龙江烟区烟草马铃薯Y病毒株系的分子鉴定

Molecular Identification of Tobacco Potato Virus Y Strains in Heilongjiang Tobacco Planting Areas

摘要: 为明确黑龙江烟区烟草马铃薯Y病毒(Potato virus Y,PVY)株系种类及其分布状况,采用多重PCR技术及测序手段,对采集黑龙江省烟叶产区的107份疑似PVY样品进行了鉴定分析。结果表明:4个有代表性的PVY分离物HLJ-SH8、HLJ-SC2、HLJ-SC6和HLJ-MDJ4的基因组序列全长分别为9 698、9 702、9 702和9 698 bp,都包含一个由9 186 bp组成的开放读码框(ORF),编码一个由3 061个氨基酸组成的多聚蛋白。系统进化树分析结果表明这4个PVY分离物分别为PVY^NTN、PVY^N、PVY^NW和PVY^NTN-NW株系,其中PVY^NTN-NW株系是侵染黑龙江烟草的优势株系。

Abstract: To clarify the kinds and distribution states of tobacco Potato virus Y(PVY) strains in Heilongjiang tobacco planting areas,107 suspected PVY isolates collected from Heilongjiang tobacco planting areas were identified with multiplex polymerase chain reaction(Multiplex-PCR) and sequencing techniques.The results indicated that the full length of genomes in four representative isolates(HLJ-SH8,HLJ-SC2,HLJ-SC6 and HLJ-MDJ4) were 9 698,9 702,9 702 and 9 698 bp,respectively.All of the four isolates contained an open reading frame(ORF) of 9 186 bp in length and encoded a polyprotein of 3 061 amino acids.Phylogenetic analysis indicated that the four isolates were PVY^NTN,PVY^N,PVY^NW and PVY^NTN-NW strains,respectively;among which,PVY^NTN-NW strain was the dominant strain in Heilongjiang tobacco planting areas.