Abstract: To investigate the Tobacco mosaic virus (TMV) resistance in new tobacco germplasm resources introduced from America, the TMV resistances of 304 new tobacco germplasm resources were evaluated by inoculating with high pressure spray gun at seedling stage via a pot experiment. The results showed that there were 94 high resistant resources, 15 resistant resources, 56 moderate resistant resources, 122 moderate susceptible resources and 17 susceptible resources. Among the 94 high resistance germplasm resources, there were 14 burley, 28 cigar, 2 air-cured, 14 flue-cured, 1 Maryland, 4 oriental and 31 Nicotiana rustica and crossing leaf varieties. The ratios of high resistant germplasm in the 7 germplasm types mentioned above were different, their ranks were Nicotiana rustica and crossing materials > flue-cured tobacco > burley tobacco > cigar tobacco > air-cured tobacco > oriental tobacco > Maryland tobacco.