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二氯甲烷提取物致香成分GC/MS指纹图谱在烤烟香型鉴别中的应用

Identification of flue-cured tobacco flavor style using GC/MS fingerprint of aroma component extracted by dichloromethane

  • 摘要: 以3种香型90份烤烟烟叶样品为研究对象,通过感官评吸确定烤烟香型。用二氯甲烷提取样品,采用VIP结合PLS-DA法分析GC/MS指纹图谱的特征信息,解析影响烤烟不同香型的致香成分,分别构建指纹图谱和致香成分的分类模型。进而分析特征致香成分与烤烟主体香韵的相关关系,探析影响烤烟主体香韵的致香成分。结果表明:①筛选出1 533个特征指纹图谱信息,建立的GC/MS指纹图谱判别模型的验证准确率、R2、RMSECV和RMSEP分别为96.67%、0.926、0.190和0.250;②解析出对烤烟不同香型分类具有较大贡献的52个致香成分,建立的致香成分分类模型的验证准确率、R2、RMSECV和RMSEP分别为86.67%、0.817、0.301和0.396;③通过逐步回归分析引入16项致香成分指标,建立烤烟香型(浓香、清香和中间香)的判别方程,其回代验证判别正确率分别为100%、91.7%和97.2%;④优选的16个特征致香成分与烤烟主体香韵均显著相关。上述判别模型均可有效鉴别不同香型烤烟,尤其是特征指纹图谱判别模型及烤烟香型逐步回归判别方程均较基于52个致香成分建立的分类模型具有更高的识别准确率。

     

    Abstract: Taking 90 flue-cured tobacco samples of 3 flavor styles as the research objects, the flavor styles of the samples were determined by sensory evaluation. The samples were extracted by dichloromethane, and the characteristics of GC/MS fingerprints were analyzed by VIP (Variable importance in the projection) method combined with PLS-DA (Partial least squares discriminant analysis). The aroma components influencing the flavor style of flue-cured tobacco were analyzed, and the classification models of the fingerprints and aroma components were built separately. The correlations between the characteristic aroma components and main aroma note of flue-cured tobacco were analyzed, and the aroma components influencing the main aroma note were investigated. The results showed that:1) 1 533 pieces of information of characteristic fingerprints were screened out, and the validation accuracy, R2, RMSECV and RMSEP of the developed GC/MS fingerprint discrimination model were 96.67%, 0.926, 0.190 and 0.250, respectively. 2) 52 aroma components greatly influencing the classification of flue-cured tobacco flavor style were identified, and the validation accuracy, R2, RMSECV and RMSEP of the developed aroma component classification model were 86.67%, 0.817, 0.301 and 0.396, respectively. 3) 16 aroma components were introduced via stepwise regression analysis, and the discrimination equations for flue-cured tobacco flavor styles (robust, fresh and medium flavor styles) were established. The back substitution verification indicated that the discrimination accuracies of these equations were 100%, 91.7% and 97.2%, respectively. 4) The selected 16 aroma components significantly correlated to the main aroma note of flue-cured tobacco. All above mentioned discriminant models could identify the flavor style of flue-cured tobacco, the accuracies of characteristic fingerprint model and stepwise regression discriminant equations were higher than that of classification model based on 52 aroma components.

     

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