Abstract: To investigate the differences of bacterial community structure among tobacco strips from different growing areas and stalk positions during aging, the bacterial 16S rDNA of strip samples of upper, middle and lower leaves from 4 areas (Youxi in Fujian, Zhanyi in Yunnan, Changshun in Guizhou and Queshan in Henan) during aging were sequenced by Illumina MiSeq. The results showed that 940 operational taxonomic units (OTUs) were obtained from 12 samples. The samples (C3F) from Youxi had the most abundant bacterial biodiversity. The dominant phyla in 12 samples were Proteobacteria, and the dominant genera in most samples were Sphingomonas. The Venn diagram of bacterial OTUs of strips from different areas during aging revealed that 195 of OTUs were common to the strips from 4 areas, the numbers of specific OTUs for Youxi, Zhanyi, Changshun and Queshan were 300, 9, 4 and 0, respectively.