Abstract: In order to investigate the systematic difference of moisture content in tobacco leaf tested in labs in areas of different altitudes above sea level, nine leaf samples were tested in eight labs. The correlation between atmospheric pressure and the measured moisture content was analyzed. The correction equation for moisture content in leaf to standard atmospheric pressure was established via LINEST regression function, and validated. The results showed that:1) The test value of moisture content in tobacco leaf negatively correlated to atmospheric pressure, their correlation coefficient R² ranged from 0.519 6 to 0.917 5; the correlations between the moisture content in the nine samples and atmospheric pressure all reached significant or extremely significant level. 2)The correlation between the moisture content and atmospheric pressure fitted a triple polynomial best. 3) The corrected test values of the samples under non-standard atmospheric pressure were compared with the values tested under standard atmospheric pressure, and the maximum deviation between them was 0.668 9%.