Abstract: To explore the effect of tobacco stalk biochar on soil respiration rate, a pot experiment was conducted to detect the respiration rate, temperature and moisture content of soil at the growth stage of tobacco. The results showed that under different fertilization treatments using the biochar, the respiration rate of tobacco-plant soil changed significantly with the development and growth of tobacco with the lowest and highest respiration rate appeared on the 60th day and the 90th day after tobacco seedling transplanting, respectively. The respiration rate of soil treated with biochar was higher than that without biochar or with conventional fertilization. The variation of soil respiration rate was well characterized by a double-factor model of soil moisture content and temperature. Temperature sensitivity coefficients of soil respiration rate of different treatments were from 2.36 to 9.41. The application of biochar reduced the sensitivity of soil respiration rate to soil temperature. Soil respiration rate of four treatments (conventional fertilization + 12 g/kg biochar, conventional fertilization + 24 g/kg biochar, conventional fertilization-5% pure nitrogen + 12 g/kg biochar and conventional fertilization-10% pure nitrogen + 24 g/kg biochar) significantly negatively correlated to cation exchange capacity(CEC). Soil pH, organic matter(except for the control, no fertilization), alkaline hydrolysis nitrogen, available phosphorus and available potassium negatively correlated to soil respiration rate. Therefore, proper biochar could increase soil respiration rate.