Abstract: To investigate the spatial distributions and influencing factors of exchangeable Ca and Mg contents and Ca/Mg ratio in soils from Wenshan tobacco-growing areas, 276 representative soil samples were collected from Wenshan with Global Positioning System(GPS)technique. The contents of exchangeable Ca and Mg in soil samples were determined, and their spatial distributions and influencing factors were studied with geostatistics and classical statistical methods. The results showed that:1) The contents of exchangeable Ca and Mg were rich in Wenshan soils, with an average value of 2 389.15 and 213.95 mg·kg^-1, respectively. However, Ca/Mg ratio was imbalanced. Physiological calcium deficiency (Ca/Mg < 5) and physiological magnesium deficiency(Ca/Mg>10) could occur in tobacco from 17.10% and 54.64% of Wenshan tobacco-growing areas, respectively. The spatial analysis suggested that exchangeable Ca and Mg contents and Ca/Mg ratio presented clear distribution characteristics. 2) The canonical correlation analysis indicated that exchangeable Ca and Mg contents and Ca/Mg ratio in Wenshan tobacco-growing soils were significantly affected by altitude, soil pH and total phosphorus in soil. The stepwise regression analysis showed that the influence of soil pH was the most significant factor. 3) The exchangeable Ca and Mg contents and Ca/Mg ratio increased with the increase of soil pH and total phosphorus content, while the exchangeable Ca content decreased with the rise of altitude.