Abstract: In order to investigate the influences of endogenic glucose in tobacco leaves on carbon monoxide (CO) release during cigarette combustion, the pyrolysis of glucose was simulated by a thermogravimetric analyzer (TG) and pyrolyzer, and the CO yield formed by pyrolysis was determined with an electrochemical method and FTIR method. Furthermore, the impacts of 0%-4.0% (mass fraction) of potassium salts (potassium citrate, potassium carbonate and potassium sulphate) addition, oxygen concentration (0%-20%, volume fraction) and heating rates (50℃/s-300℃/s) were also studied. The results showed that:1) With oxygen-assisted pyrolysis under slow temperature ramp (10% of O₂, 20℃/min), CO formation from glucose could be divided into a pyrolysis stage and a carbon residue oxidation stage, which corresponded with the two stages of mass loss curve. The CO yield in the presence of oxygen was about twice as high as it was under the inert condition and the slow programmed temperature. 2) CO yield and decomposition temperature of glucose were reduced by potassium citrate in an apparent dose-response relationship. 3) Under air atmosphere and fast heating (50℃/s-300℃/s), the CO yield increased with the increase of heating rate. 4) Under air atmosphere and at a heating rate of 300℃/s, CO yield was reduced by potassium citrate and potassium carbonate. However, the potassium sulphate had no noticeable effect on the CO yield from glucose.