Abstract: To evaluate the influences of different puffing parameters on TPM delivery in aerosol of e-cigarettes, the TPMs released from e-cigarettes were collected under ISO and Health Canada Intensive (HCI) smoking regimens, using 3 puffing profiles consisting of puffing time of 2-5 s per puff and 1-100 puffs. Three types of e-cigarette were used in this study: two were rechargeable and one was disposable. The results showed that: 1) The major factors affecting the TPM delivery were puff time and the TPM releasing rate of e-cigarettes, while the effects of the other puffing parameters such as puffing duration were slight. 2) Fast puffing air flow tended to decrease the working temperature of electric heating coil in the cartridge of the e-cigarette, sometimes even below the boiling point of glycerol, which resulted in low smoke delivery. 3) The TPM delivery of e-cigarette decreased gradually with the proceeding puffs. Therefore, the appropriate smoking regimen for the aerosol analysis of e-cigarette was puffing time 3-4 s, rectangular puffing profile, puff volume 55 mL and collecting the TPM of 1-30 puffs.