Abstract: AtPAP1(Arabidopsis thaliana production of anthocyanin pigment 1)is a key regulatory gene in the anthocyanin biosynthetic pathway. To achieve the accumulation of AtPAP1 gene in tobacco, glandular-trichome specific expression promoter proCYP71D16 was used to construct pCAMBIA1391-CYP71D16-PAP1 vector. pCAMBIA1391-35S-PAP1 and pCAMBIA1391-CYP71D16-PAP1 vectors were transferred into tobacco with Agrobacterium-mediated method. Transgenic plants were detected by PCR analysis in DNA and RNA levels and the phenotypes were observed. Furthermore, the contents of anthocyanin in transgenic plants were determined. The results showed that the 35S::AtPAP1 plants exhibited red leaf surface, green glandular trichome cell and colorless glandular stem cell. The CYP71D16::AtPAP1 plants exhibited green leaf surface, however, the head of glandular trichome was pink. The control plants possessed green leaf and glandular trichome. HPLC data indicated that cyanidin and pelargonidin accumulated in 35S::AtPAP1 plants; only a little of cyanidin accumulated in CYP71D16::AtPAP1 plants, while anthocyanin was not detectable in the control plants. This study achieves the expression of AtPAP1 in tobacco and provides a theoretical basis for studying the material metabolism in tobacco.