Abstract: In order to investigate the adsorption characteristics of tobacco materials (cut leaf strip, cut reconstituted tobacco and cut stem) in a cigarette for volatile organic compounds (VOCs), a headspace-gas chromatography-mass spectrometry (HS-GC/MS) method was developed to determine 10 VOCs (benzene, toluene, ethylbenzene, styrene, acetone, butanone, ethyl acetate, propyl acetate, isopropyl acetate, butyl acetate) adsorbed by these tobacco materials. The amounts of VOCs adsorbed by different tobacco materials at different VOC concentrations during different adsorption time were determined, and the variations of amount of VOCs adsorbed by the tested samples along with their storage duration in a workshop were analyzed. The results showed that: 1) The optimized headspace equilibrium condition was 90℃ for 40 min. 2) The VOCs were quantified by an external standard method with the good linearity (R²>0.99) of calibration curves. The recoveries were 85.6%-102.1% with the relative standard deviations ranged from 2.4% to 6.4%. The limits of detection and quantification for the 10 VOCs were 0.01-0.04 and 0.03-0.13 μg/g, respectively. 3) The amount of VOCs adsorbed by cut reconstituted tobacco was the highest, while that by cut leaf strip was the lowest. Basically, the adsorption amount increased gradually with the increase of adsorption time and VOC concentrations in the environment. 4) Trace amount of several VOCs were detectable in the tobacco samples from newly built workshop, however the adsorbed VOCs were desorbed gradually with the prolongation of storage in clean air.