Abstract: In order to evaluate the potential of tobacco stems and develop a new e-cigarette flavor, supercritical fluid CO₂ extraction technology was employed to extract aroma components in tobacco stems. The chemical components in the extract were analyzed by GC/MS, and the flavoring effect of the e-cigarette liquid was evaluated by sensory evaluation of the aerosol produced. The results showed that: 1) The optimal extraction parameters were: extraction pressure 20 MPa, extraction CO₂ flow 5 L/min, extracting for 30 min at 120℃, and the yield was 9.9% of the stem by weight under such condition. 2) GC/MS identified 32 aroma components in tobacco stem; among them, the content of 2,3-dihydro-3,5-dihydroxy-6-methy-4H-pyran-4-one (DDMP) was higher, which was believed to mainly contribute to the roasted and tobacco-like aroma of the e-cigarette aerosol. 3) The obtained tobacco stem extract was shown to impart sweet, roasted and tobacco-like aroma to e-cigarette, and thus had the potential to aid the development of e-cigarettes of tobacco characteristic styles.