Abstract: Desktop scanning electron microscope(SEM)was employed to determine special fibers in cigarette paper, and key parameters were researched. The results indicated that:1) Magnification of 400 to 500 was appropriate for full view analysis of samples, that for micro-morphology analysis and fiber type identification was 1 000 to 2 000. Both backscattered electron detector and secondary electron detector were suitable for the determination of special fibers in cigarette paper; the appropriate accelerating voltage ranged from 5 to 15 kV; and it was not necessary to set the imaging parameters, test area and size specifically. 2) The collaborative validation of the method was conducted with 10 samples in 10 laboratories, and the results of desktop SEM method well agreed with those of current standard method. 3) Comparing with current standard methods, the desktop SEM method shortened the determination time by more than 75 minutes, and the test magnification and efficiency promoted remarkably.