Abstract: In order to accurately determine the moisture content in an unused cigarette filter rod, a gas chromatography-thermal conductivity detector (GC-TCD) method was established by using acetonitrile as internal standard and isopropanol as extraction solvent for oscillation extraction. The test results of the developed method were compared with those measured using a standard method (the oven method). The results showed that:1) The limit of detection, recoveries, relative standard deviations (RSDs) of the developed method was 0.03%, 96.5%-108.3%, 0.32%-3.07%, respectively. 2) When filter rod was free of triacetin (glycerol triacetate, GT), the results obtained by the two methods were basically the same. However, the result of oven method was about twice as high as that of GC-TCD method if filter rods contained triacetin. 3) The moisture contents of filter samples, include one acetate fiber filter rod and ten polypropylene fiber tow filter rods, by GC-TCD method were 1.88% and 3.65%-6.17%, respectively, which were lower than the required limit. This method solves the issue of relative higher moisture contents during using the oven method caused by the thermal volatilization of triacetin in filter rod, the detection result is more accurate and reliable. The method is simple, sensitive, and suitable for determining the moisture content in different cigarette filter rods.