Abstract: To investigate the effects of organic manure on soil carbon sequestration and CO₂ emission reduction in tobacco planting field, a field experiment was conducted to study the effects of different fertilization treatments on soil organic carbon composition, CO₂ emission flux and carbon pool management index. The results showed that:1) Comparing with applying chemical fertilizer only, the application of chemical fertilizer and organic fertilizer could increase the contents of total organic carbon and high, medium and low active components, and improve the ratio of soil carbon to nitrogen. The content of total organic carbon and the ratio of carbon to nitrogen increased by 11.44% and 0.75, respectively. Increasing organic fertilizer significantly improved the activity, index, activity index and management index of soil carbon pool, and effectively promoted soil quality. 2) Under the condition of fertilization, tobacco soil ecosystem was a weak carbon sink, the application of organic fertilizer and chemical fertilizer increased the carbon sequestration of tobacco plants by 93.63 kg·hm^-2 comparing with chemical fertilizer only. The application of organic fertilizer promoted soil CO₂ emission, significantly increased soil organic carbon content and tobacco yield, and reduced greenhouse gas emission intensity. Therefore, increasing organic fertilizer in tobacco planting fields is an important way to promote tobacco yield, carbon sequestration and emission reduction.