Abstract: To investigate the effects of grafting on the growth, development, yield, chemical components and black shank resistance of flue-cured tobacco and its feasibility in flue-cured tobacco production, field experiments were conducted by taking high resistant cultivar as a rootstock and quality cultivar as a scion. The growth, development, yield and quality of rootstock-grafted tobacco seedlings in fields were investigated. The reciprocal grafting among cultivars of different black shank resistance was carried out and the black shank resistance in different grafted combinations was identified in disease nursery. The results showed that grafted seedlings had an obvious healing period after transplant. At flower-bud appearing stage, the growth and biological characteristics of grafted tobacco were comparable to scion, and their difference was not obvious within the growth period in fields. The yield and chemical component indexes of grafted tobacco were between those of the rootstock and scion, some grafted combinations presented heterobeltiosis. The identification results in disease nursery showed that the black shank incidence rates of the graft combinations, in which the cultivars of high black shank resistance used as the rootstock or scion were grafted with susceptible cultivars, decreased significantly, especially when high resistant cultivars were used as the rootstock. Therefore, grafting with suitable rootstock and scion is an efficient means to control tobacco black shank.