Abstract: To accurately characterize the cigarette smoke dose in whole smoke exposure experiments, quartz crystal microbalance (QCM) and gas chromatography-mass spectrometer (GC/MS) were employed to determine cigarette smoke dosimetry based on whole smoke exposure. QCM was used to accurately measure the mass concentrations of smoke particulate matter deposited on the surface of QCM in a whole smoke exposure module in real time. The concentration of nicotine in the deposited particles was analyzed by GC/MS as a marker for key particulate phase components. The results indicated that:1) QCM technique could be applied to the determination of in vitro particulate dosimetry under whole smoke exposure situation; 2) the results of nicotine concentration was well correlated to the mass concentration of smoke particulate matter (R²>0.85), and nicotine could be selected as a chemical marker for accurate particulate phase smoke dose characterization.