Abstract: In order to develop novel cigarette flavors that can promote the aroma quality of cigarettes and the stability of aroma-releasing, rhodinol and rhodinol glycoside were added into cigarettes, and their transfer rates to mainstream cigarette smoke were measured. The sustained release of glycoside was analyzed through puff-by-puff smoking, and the volatile aroma components were determined by GC/MS. Furthermore, the sensory quality of cigarettes containing rhodinol or rhodinol glycoside was evaluated. The results showed that:1) The transfer rate of rhodinol produced by rhodinol and rhodinol glycoside to the particulate phase matter of mainstream cigarette smoke was 10.80% and 2.21%, respectively. 2) In the process of puff-by-puff smoking of test cigarettes, 31 volatile products were detected. The aroma profile of cigarettes containing rhodinol was not uniform, the release was faster in the first puff and then slowed. On the other hand, the aroma of cigarettes containing rhodinol glycoside released uniformly. 3) The aroma of cigarette containing rhodinol glycoside was rich and consistent during smoking, which suggested that rhodinol glycoside could promote and modify cigarette smoke and reduce irritation. Rhodinol glycoside avoids instable aroma release caused by the volatilization of rhodinol, and could be used as a raw material in cigarette flavoring.