Abstract: To investigate the genetic diversity of Ralstonia solanacearum and effectively control tobacco bacterial wilt, 33 strains were isolated from Yihuang, Lichuan, Le'an, Guangchang, Zixi and Nanfeng in Fuzhou tobacco-planting areas and identified by PCR with primer 759/760. The genetic diversity of the 33 stains was analyzed by McRAPD and sequence of endoglucanase gene (egl) and the sequevar type of those strains were confirmed. McRAPD results showed that geographical origin influenced the genetic diversity of most strains slightly. Sequencing results indicated that the 33 strains belonged to the sequevars (13, 15, 17 and 34) from Asian phylotype I and the sequevar closely corrected to geographical origin. Sequevar 15 (including 19 strains) was most abundant, accounting for 57.6%, which was isolated from samples from 10 towns in 6 counties including Guangchang, Lichuan, Le'an, Nanfeng, Yihuang, Zixi, etc., therefore sequevar 15 was the dominant sequevar. The proportion of sequevar 17 (including 3 strains) was the lowest, accounting for 9.1%, which was isolated from Huangpi Town in Yihuang County. Sequevar 34 (including 4 strains) accounted for 12.1%, which was isolated from Zengtian Town in Le'an County, Tanxi Town in Lichuan County and Gaofu Town in Zixi County. Sequevar 13 accounted for 21.2%, which was isolated from Yunyan 87 planted in Jianfeng Town and Toupi Town in Guangchang County and Fufang Town and Taiyuan Town in Nanfeng County. It was the first time that sequevar 13 was found in Fuzhou.