Abstract: To investigate the effects of different curing methods on pesticide residues in tobacco, HPLC-LTQ Orbitrap MS method was used to analyze the residues of pymetrozine, imidacloprid, cyantraniliprole, λ-cyhalothrin, dimethachlon, methyl-thiophanate, butralin and maleic hydrazide on flue-cured and air-cured tobacco. The contents of pesticide residues in cured tobacco leaves were calibrated by calcium ion content. The results showed that according to the variation of calcium ion content, the contents of dry matter in tobacco leaves were massively consumed by 29.0% and 52.2% during flue-curing and air-curing, respectively. The residues of the eight pesticides in tobacco leaves degraded in both curing processes, however different curing methods had different effects on the content of pesticide residues. The degradation rates of the eight pesticides were 28.1%-57.9% and 45.1%-72.1% during flue-curing and air-curing, respectively. The degradation rates of residual pesticides in air-cured tobacco leaves were higher.