Abstract: For quantifying the total content of octyl and decyl glycerate (ODO) mixture in breakable flavor capsule for cigarette filter, a gas chromatography-flame ionization detector (GC-FID) method based on relative response factor principle was developed. One crashed capsule was vortex extracted with n-hexane, the components of ODO were analyzed by GC-FID. Based on the concept of effective carbon number (ECN), the relative response factor (RRF) for each component of ODO was estimated, and the quantification of total content of ODO mixture was achieved successfully by using caprylin or decanoin as calibration reference materials (RMs). The results showed that:1) Each component in ODO was well separated within 20 min. 2) Taking caprylin or decanoin as the calibration reference material, RMs had a good linear relationship (R²>0.997) in the range of 0-0.5 mg/mL. The recoveries of total content of ODO ranged from 98.0% to 108.0% with relative standard deviations no more than 2.0%. The LOQ of each component in ODO was 0.05 mg/mL. 3) The ODO content in breakable capsules in 8 commercial samples of different flavoring types was from 23% to 87%. Among the examined components in ODO, the highest component was triglyceride, which accounted for more than 93% of the total ODO content. The lowest component was diglyceride that accounted for about 6%. The ODO amount of all the samples quantified by using caprylin as the calibration reference material was consistent with that quantified by using decanoin. This method is simple, efficient and suitable for the determination of total content of ODO mixture in breakable capsules.