Abstract: In order to study leaf chemical composition for tobacco leaf sectioning, leaf samples of robust flavor style flue-cured tobacco from Henan Province were cut into 10 portions (HN-1 to HN-10) from tip to base. The differences in volatile organic acid contents in different leaf portions were investigated with a single-factor variance analysis and correlation regression analysis to build leaf portion clusters according to their volatile organic acid contents. The results showed that:1) The variations in the total volatile organic acid contents from the tip to the base from different varieties and from different planting areas were basically the same, except that the total content of volatile organic acids in the portion of HN-1 and HN-5 reached a significant or extremely significant level between the different planting areas. There were no significant difference between different tobacco varieties. 2) The contents of acetic acid, butyric acid, isopentanoic acid, pentanoic acid, and the total content of volatile organic acids extremely significantly differed between the different portions. 3) The contents of acetic acid, propionic acid, butyric acid, isopentanoic acid, pentanoic acid, nonanoic acid and the total content of volatile organic acids significantly or extremely significantly positively correlated to the sectioned portions:from leaf tip to base, nonanoic acid content presented a linear increasing trend, and the contents of acetic acid, propionic acid, butyric acid, isopentanoic acid, pentanoic acid and the total content of volatile organic acids decreased first and then increased. 4) The 10 sections were clustered into 3 groups, the total content of volatile organic acids in the tip group (HN-1 to HN-5), the middle group (HN-6 to HN-8) and the base group (HN-9 to HN-10) were 227.23, 282.56 and 430.48 μg/g, respectively. 5) The sensory evaluation indicated that the scores of aroma quantity, mildness, aftertaste, offensive odor and irritation, and the total score of sensory evaluation differed significantly or extremely significantly between the 3 groups, and the total score of sensory evaluation was ranked as the tip group > the middle group > the base group.