Abstract: In order to clarify the spatial-temporal variability of available boron (B) in tobacco-planting soils of paddy-upland rotation fields in southern Hunan, soil samples were collected from Chenzhou tobacco-planting areas in 2000 and 2015 separately. The contents of available B in soils were determined, and the spatial-temporal variability of available B and its influence factors were analyzed by classical statistics and geostatistics methods. The results from statistical characteristics showed that, after continuous application of B fertilizer in Chenzhou tobacco-planting areas for 15 years, the average content of soil available B increased from 0.17 mg/kg in 2000 to 0.55 mg/kg in 2015, an increase of 223.53%. On spatio-temporal distribution, the areas with "moderate" and "high" available B contents in 2015 significantly increased by 80.49% and 19.51%, while those with "extremely low" and "low" available B contents significantly decreased by 67.13% and 32.87%, respectively. The available B content in soils was closely related to landform, topography, soil parent material, soil pH and soil organic matter.