Abstract: In order to investigate the releasing behaviors of aroma components in aerosol collected matter (ACM) from heat-not-burn (HNB) tobacco products, reconstituted tobacco particles (RTPs) with various contents of glycerol as the raw materials were heated between 150-350 oC by a bespoke HNB device simulator. The aroma components in the ACM were determined by heart-cut dimensional gas chromatography-mass spectrometry. The results showed that:1) A total of 74 aroma components were identified when heated at 350℃. 2) The total release of aroma components increased by raising heating temperature or adding glycerol to the raw materials. Heating temperature was the main influencing factor above 300℃, while glycerol was the main influencing factor below 300℃. 3) The varying releasing trends of aroma components in different categories along with the variations of heating temperature and glycerol amount were different. The overall trend was that raising temperature and adding glycerol would increase the releases of aroma components to a different extent, while the releases of furans and alkenes compounds increased first and then decreased with the rise of heating temperature. 4) Tobacco alkaloids were the main constituents of aroma components at heating temperatures below 180℃. With gradual rises in heating temperatures, tobacco alkaloids, organic acids and furans compounds were the main components released. Tobacco alkaloids, phenolic compounds, organic acids, and carbonyl compounds were the main components at heating temperatures above 300℃. The proportions of nitrogen-containing heterocyclic compounds, olefin, esters and alcohol compounds were relatively low, in which the proportion of esters were the lowest.