Abstract: In order to establish a more accurate weighting method for flue-cured tobacco quality evaluation, tobacco leaf samples were collected from 11 bases of China Tobacco Jiangsu Industrial Limited Corporation in Yunnan Province. Single and comprehensive weighting methods were used to evaluate tobacco leaf quality and the results of the weighting and evaluation were compared. The results showed that:1) According to the quality scores of the tobacco leaves from the different planting areas, their color, maturity, leaf structure and leaf body were satisfactory; their oil and color intensity were slightly weaker; the reducing sugar and sugar/nicotine ratio were relatively high; their total sugar, nitrogen, chlorine and nitrogen/nicotine ratio were appropriate, and the total alkaloid, potassium content and potassium/chlorine ratio were relatively lower. The scores on aroma quality, aroma quantity, volatility, smoothness, mildness, mellowness and aftertaste were close; the offensive odor was relatively less, and the average scores on irritation and dryness were close. Among the quality indexes, the biggest and smallest standard deviations were 5.76 for reducing sugar; and 0.16 for aftertaste, respectively. 2) For the three single weighting methods, the highest weighting coefficients were 0.086 for aroma quality in AHP, 0.119 for reducing sugar in the standard deviation method and 0.251 9 for sugar/nicotine ratio in the entropy method; and the average of variance (R²_mean) was 16.67. 3) The highest and lowest weighting coefficients of the three comprehensive weighting methods were 0.184, 0.162, 0.138 for sugar/nicotine ratio, and 0.006, 0.008, 0.010 for chlorine content, respectively; and the average of variance (R²_mean) was 1.33. 4) Among the three comprehensive weighting methods, the difference of scores evaluated by the method based on the sum of deviation squares was relatively higher, the evaluation results of the method based on the game theory had better correlations, and the evaluation score of the method based on units of constraints was relatively higher. Comparing with the signal weighting methods, the three comprehensive weighting methods had less weighting differences, more identical evaluation results, better convergence and higher coincidence with the actual situation. Therefore, the three comprehensive weighting methods are more suitable to weigh different quality indexes in the quality evaluation of flue-cured tobacco leaves.