Abstract: To objectively evaluate the effect of cigarette smoke on sensory comfortableness, an oral bionic absorption device to simulate exposure of mainstream cigarette smoke was developed. The results from the device taking six phenols and nicotine as analytes were compared with the actual cigarette smoking by volunteers. The capture of six phenols and nicotine in mainstream cigarette smoke by the bionic smoke absorption liquid and Cambridge filters were compared and their correlations with sensory comfortableness were analyzed. The results showed that:1) At 95% confidence level, there was no significant difference between the deliveries of nicotine and 5 phenols in mainstream cigarette smoke as determined by the bionic device and the t-test of volunteers' saliva. 2) For the 8 cigarette samples tested and using phenols as an example, the phenol contents in the smoke bionic absorption liquid of the bionic device was only 0.03%-23.75% of that captured by the Cambridge filter, and its correlation with sensory physiological reaction of human was more significant. Therefore, the bionic absorption device could analyze the chemical components in smoke exposure much objectively, which could provide technique support for cigarette smoke analysis.