Abstract: To investigate the control efficiency of combinatory antagonistic bacteria FP246 against tobacco black shank and its influence on the functional variations of soil microbial community, a pot experiment was conducted and the functional diversity of rhizosphere soil microbial community was analyzed by Biolog-ECO after FP246 treatment. The results showed that the incidence of tobacco black shank was reduced by FP246 treatment with the control efficiency of 85.40%. No significant difference was found in Shannon-Wiener diversity index between FP246 treatment (T1) and that treated by a 58% Fubol wettable powder (T3), while the differences from 10⁹ spore/g Bacillus subtilis wettable powder treatment (T2) and the control (T4) were statistically significant. The McIntosh index rank and the richness index was in the order of T3 > T1 > T2 > T4. Simpson's dominance indexes did not significantly differ among T1, T2 and T3. Comparing with the control, the AWCD values in T1, T2 and T3 were significantly increased and significantly differed from that of the control. A principal component analysis indicated that carbon source utilization of microorganisms was higher from 96 to 144 h, especially sugars, amino acids, carboxylic acids and polymers.