Abstract: BELL genes, a ubiquitous class of genes in plants and encoded transcription factors with homeobox domains, involve in regulating the development of many plant tissues, such as leaves, inflorescences, etc. To clarify the structure and function of tobacco BELL genes, NtBELL were firstly identified by homology comparison and functional domain analysis. The functions of NtBELL were preliminarily predicted by phylogenetics and gene expression pattern analysis. The results showed that 28 NtBELLs were identified in the genome of Nicotiana tabacum and distributed on 15 chromosomes separately. Phylogenetic analysis indicated that NtBELLs could be divided into four groups and each group participated in regulating different developmental processes. NtBELL had different expression patterns in six tobacco tissues and presented apparent tissue specificity. Drought and salt stress significantly induced the expressions of some NtBELLs, which suggested that BELL gene family might be involved in resisting abiotic stress in plants.