Abstract: To clarify the occurrence of major tobacco pests in Zunyi tobacco fields and select a suitable formula using sugar-acetic acid mixtures, five formulas of sugar-acetic acid mixtures were used to trap and kill tobacco pests in Zunyi tobacco-growing area from April 17 to August 15 in 2017. The results showed that the occurrence of adult Agrotis ipsilon and Helicoverpa assulta/Helicoverpa armigera peaked in early June and early May respectively, and other moths had two obvious peaks in early June and early July. The peaks of Popillia quadriguttata adults appeared in late May to early June and in early July. The sugar-acetic acid mixtures caused little harm to natural insect enemies, but was more harmful to bees after tobacco budding. Among the five formulas of sugar-acetic acid mixtures, the formulaⅠ(sugar:vinegar:ethanol:water=6:3:1:10) had the best trapping and killing effect. Its harm on bees was relatively smaller. The results also indicated that the pest-eaten rate of tobacco leaves in the experimental areas was significantly higher than that in the control areas at the mature stage of tobacco.