Abstract: In order to reduce the application amount of chemical fertilizer and improve the utilization efficiency of chemical nutrients in flue-cured tobacco-planting areas of southern Anhui Province, the effects of topdressing frequency of water-soluble fertilizers on nutrient uptake and leaf quality were studied by reducing nitrogen, phosphorus and potassium fertilizers at 10%, 30% and 30% respectively. The results showed that after three times of topdressing, the leaf accumulation amount of nitrogen, phosphorus and potassium were significantly decreased by 14.4%, 8.5% and 15.5% respectively when compared with the conventional fertilization method. The leaf quality was significantly improved. Topdressing at four times could increase the apparent utilization rates of phosphorus and potassium fertilizer by 3.2 and 15.0 percentage points respectively, however the leaf quality was significantly lower than that under three times. The apparent utilization rates of nitrogen, phosphorus and potassium were significantly increased by 12.8, 2.9 and 17.9 percentage points respectively with topdressing for five times, but the leaf quality was lower. Meanwhile, increasing the proportion of ammonium nitrogen in water-soluble fertilizers could improve the utilization efficiency of chemical nutrients and the quality of flue-cured tobacco leaves.