Abstract: In order to effectively control tobacco bacterial wilt, cell culture and capillary methods were used to investigate the influences of tobacco allelopathic auto-toxic substance dibutyl phthalate on the growth of R. solanacearum and the chemotaxis-inducing effects. In addition, a gradient acclimation method was used to screen dibutyl phthalate degrading bacteria, and the incidence of tobacco bacterial wilt disease after the treatment was measured by pot experiments. The results showed that dibutyl phthalate had a chemotaxis-inducing effect on R. solanacearum and could promote the growth of R. solanacearum when applied at a low concentration. The degradation rate of the screened dibutyl phthalate degrading bacterium (Aicontobacter sp. Ed2) was 51.17% in soil. The pathogen amount of R. solanacearum in the soil treated with dibutyl phthalate (T1) significantly increased by 59.89% compared with the CK, while those in the soil treated with Ed2 (T2) decreased by 84.63% and 90.38% respectively compared with the CK and T1. Therefore, using dibutyl phthalate degrading bacteria to degrade dibutyl phthalate in soil decreased the nutrition supply of R. solanacearum and restrained the chemotaxis-inducing effect of dibutyl phthalate on R. solanacearum, thereby the incidence of tobacco bacterial wilt was effectively decreased.