Abstract: In order to clarify the affecting mechanism of root irrigation with 3-methylbenzothiazole-2-thione secreted by corn roots on the community structure of bacteria in the rhizospheric soil of tobacco plants infected by bacterial wilt, Illumina MiSeq high-throughput sequencing technology was used to analyze the community structure of bacteria in the rhizospheric soil of tobacco plants infected by bacterial wilt on the day of the root irrigation as well as the 2nd, 7th, 20th and 40th days after root irrigation through pot experiments. The results showed that:1) There was no obvious bacterial wilt symptom 40 days after root irrigation with 3-methylbenzothiazole-2-thione, while all tobacco plants withered and died 40 days after root irrigation with sterilized distilled water. 2) After root irrigation with 3-methylbenzothiazole-2-thione, Sobs index, Chao1 index and Shannon index of Alpha diversity indexes of bacteria in rhizospheric soil of tobacco plants increased. 3) The dominant phyla were Actinobacteria, Proteobacteria and Chloroflexi and the dominant genera were Pseudarthrobacter, Sphingomonas and Gemmatimonas in all the five stages after irrigation with 3-methylbenzothiazole-2-thione. 4) Root irrigation with 3-methylbenzothiazole-2-thione increased the relative abundances of Actinobacteria, Nocardioides and Streptomyces and decreased the relative abundances of Saccharacteria and Sphingomonas in the rhizospheric soil of tobacco plants. Therefore root irrigation with 3-methylbenzothiazole-2-thione can effectively control tobacco bacterial wilt, affect the community structure of bacteria in the rhizospheric soil of flue-cured tobacco, and increase the relative abundances of beneficial bacteria.