Abstract: In order to investigate the salt tolerances of different tobacco germplasms and to select salt tolerant tobacco cultivars, different tobacco germplasms at germination stage cultured in an artificial climate chamber were comprehensively evaluated by principal component analysis and cluster analysis. The results showed that salt stress at 150 mmol/L NaCl resulted in a 50% decrease in K326's root length. Under the treatment of 150 mmol/L NaCl, 34 tobacco germplasms presented significant differences in germination potential, germination rate, germination index, vitality index and root length. Through principal component analysis and Euclidean distance clustering, the germplasm resources were divided into four grades:high salt tolerant, salt tolerant, sensitive and high sensitive. High salt tolerant germplasms were oriental tobacco Basma. Salt tolerant germplasms included flue-cured tobacco K326, Yunyan87 and burley tobacco DS26, va1061, va509. Sensitive germplasms included flue-cured tobacco Spt168, Zhongyan100, NC89, Qinyan97, burley tobacco KY907, TN86, va1048, TN90, and oriental tobacco Samsun and Izmir. High sensitive germplasms were flue-cured tobacco NC82, K399, Jiyan9, RG11, Qinyan96, NC55, NC567, NC95, Zunyan6, K394, Yanyan97, K358, Honghuadajinyuan, Hicks, K346, coker176, CV87 and burley tobacco va1052.