Abstract: In order to establish the relationship between leaf color difference and plastid pigment in redried strips of different sizes, the threshed strips of Hongda C3F and K326 B3F tobacco leaves were sieved by a vibratory screen. The color difference parameters, L^* (lightness value), a^* (redness value), b^* (yellowness value), △E (total color different) and the contents of plastid pigments (lutein, β-carotene) in redried strips of different sizes were measured and used to construct Pearson correlation and functional relationships between the color difference and plastid pigment contents. The results showed that:1) The redried strips had significant differences in the values of L^*, a^*, b^*, △E, and also the contents of lutein, β-carotene. 2) With the decrease of strip size, the value of L^* decreased first and then increased; the values of a^*, b^*, △E and the contents of two plastid pigments increased first and then decreased, and the inflection points of the variations were in the small strips for the four parameters and the two plastid pigment contents. Among them, the L^* value of the small strips was the lowest, while the a^*, b^*, △E values and the two plastid pigment contents were the highest. The L^* value of tobacco dust was the highest, while the a^*, b^*, △E values and two plastid pigment contents were the lowest. 3) The contents of lutein, β-carotene and their total content significantly or extremely significantly negatively correlated to L^* value, and significantly or extremely significantly positively correlated to a^*, b^*, △E values. Regression analysis was carried out between the color difference parameters and the total content of plastid pigments, there was a highly fitting linear relationship between L^*, a^*, b^*, △E values and the total plastid pigment content. For the tobacco leaves of the same grade, color difference and plastid pigment contents differed greatly among the redried strips of different sizes.