Abstract: To investigate the influences of puffing parameters on the physical properties and deposition in human respiratory tract of electronic cigarette (e-cigarette) aerosols, e-cigarettes were smoked by simulating human smoking under six puffing regimes at different puff durations (2, 3, 4 s), puff volumes (35, 55, 75 mL) and puff intervals (30, 60 s). The results showed that:1) With the increase of puff duration, the particle number concentration and particle size of the aerosols raised significantly, which further increased the amount of aerosol deposition in human respiratory tract. 2) At a higher puff volume, the particle number concentration of the aerosols increased, while the particle size decreased; and the deposition fraction and deposition particle number concentration increased, while deposition particle mass concentration slightly decreased. 3) Puff interval slightly influenced the physical properties and deposition in human respiratory tract of e-cigarette aerosols.