宏转录组测序揭示褐土脲酶基因的表达丰度和细菌来源

胡利伟; 轩贝贝; 戴华鑫; 郭建华; 牟文君; 刘阳; 翟振; 闫鼎; 蔡宪杰; 奚家勤; 薛超群; 宋纪真

doi:10.16135/j.issn1002-0861.2019.0538

宏转录组测序揭示褐土脲酶基因的表达丰度和细菌来源

Expression abundances of urease genes and microbe sources of urease in cinnamon soil revealed via metatranscriptomic sequencing

摘要

摘要: 为明确褐土中脲酶编码基因的表达丰度，通过土壤RNA提取和宏转录组测序法，筛选RNA水平上表达较高的脲酶基因，共筛选到122个脲酶结构基因（93个ureC、16个ureB和13个ureA）和脲酶辅助基因115个（29个ureD、12个ureE、36个ureF、29个ureG、5个ureH和4个ureJ）。其中ureB、ureA、ureG、ureH和ureJ所有转录本TPM值都小于2，而ureC、ureD、ureE和ureF丰度最高的转录本TPM值分别为3.17、5.37、2.01和2.71。细菌来源的芽孢杆菌属Bacillus、芽孢八叠球菌属Sporosarcina、节杆菌属Arthrobacter、链霉属Streptomyces和类芽孢杆菌属Paenibacillus等在褐土脲酶活性的发挥中贡献度较大。

Abstract: Previous studies showed that the urease genes responsible for urea degradation in cinnamon soil were from bacteria, fungi and archaea. To clarify the expression abundances of urease genes, soil RNA extraction and metatranscriptomic sequencing were performed to screen urease genes with high expressions in RNA level. A total of 122 structural genes(93 ureC, 16 ureB and 13 ureA)and 115 accessory genes(29 ureD, 12 ureE, 36 ureF, 29 ureG, 5 ureH and 4 ureJ) were identified. The expression values of ureB, ureA, ureG, ureH and ureJ transcripts were below 2 in TPM (Transcripts per Million), however the highest expression values of ureC, ureD, ureE and ureF were 3.17, 5.37, 2.01 and 2.71 in TPM respectively. Bacillus, Sporosarcina, Arthrobacter, Streptomyces and Paenibacillus played essential roles in the degradation of urea in cinnamon soil.

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