Abstract: Trihelix transcription factors are a class of important transcription factors that regulate plant growth and development, and play important roles in the plant resistance to various stresses. In order to investigate Trihelix transcription factors in tobacco, a Trihelix transcription factor gene was cloned from tobacco by gene homologous cloning method and named as NtGT-2. Bioinformatics analysis showed that NtGT-2 gene belonged to the GT-2 subfamily of Trihelix transcription factors, and its maximum open reading frame (ORF) was 1 176 bp, which encoded a total of 391 amino acids. The molecular weight of NtGT-2 was predicted to be 45.01 kDa with an isoelectric point of 5.80. The secondary structure analysis indicated that NtGT-2 protein contained a typical Trihelix family structure domain (SANT structure domain) and a curly spiral structure domain (Coiled-coil structure domain). The results of real-time fluorescence quantitative PCR showed that the expressions of NtGT-2 in different tissues of tobacco were different, and the expression level was higher in stalk nodes, stalks and axillary buds and lower in leaves and roots. NtGT-2 had different responses to abscisic acid (10 μmol/L ABA), salt stress (200 mmol/L NaCl), low temperature (4℃), and 15% PEG6000 treatment, which suggested that NtGT-2 could play an important role in tobacco resisting abiotic stress.