Abstract: In order to clarify the sensory effect of single flavor on cigarettes, 100 kinds of single flavors were selected, and the role and effects of flavors in reference cigarettes were investigated by the sensory quality evaluation and the labeled affective magnitude scale method, with the evaluation results analyzed by factor analysis. The results showed that:1) In the reference cigarettes, most of the selected single flavors could improve the sensory indicators, including aroma quality, aroma volume, mildness, smoothness, volatility and concentration, while the improvement of irritancy, aftertaste and offensive odor was not obvious. 2) Three main factors, namely aroma amount factor, aroma quality factor, and comfort factor, could be generalized from the nine original indices through the factor analysis method. 3) According to the comprehensive factor score and the individual factor score ranking, it could be concluded that vanilla extract, jujube tincture, isoamyl acetate and other monomer flavors have higher comprehensive use value in reference cigarettes, while vanilla extract, 2, 3-butanedione and jujube tincture showed the individual advantages of improving the sensory quality of reference cigarettes in terms of aroma amount, aroma quality and comfort degree, respectively.