Abstract: To investigate the smoking behavior of cigarette smokers and disposable e-cigarette users, a self-matching method was adopted. A total of 66 volunteers who smoked cigarettes were recruited and randomly divided into two groups(GroupⅠ and GroupⅡ). The two groups of volunteers were switched to smoke two types of e-cigarettes with different nicotine concentrations (12 and 20 mg/mL), and their general smoking characteristics were collected via questionnaires. CReSS Pocket was used to record the smoking parameters for smokers and e-cigarette users. The contents of total nicotine metabolites and cotinine in volunteers' urine were analyzed by HPLC-MS/MS, and the test results were corrected by urine creatinine. The results showed that:1)The mean puffing volume of the two groups for cigarettes were(50.3±17.2)and(47.9±20.6)mL, puffing duration times were (1.8±0.7) and (1.7±0.7) s, puffing intervals were (14.1±9.5) and (14.9±11.7) s, respectively. The mean puffing volumes of the two groups for e-cigarettes were(57.6±23.1)and(51.1±23.1) mL, puffing duration times were(1.9±0.7)and(1.7±0.7)s, puffing intervals were(9.4±9.3)and(11.0±9.3)s, respectively. 2)The contents of total nicotine metabolites in the urine of the two smoker groups were(6 641.6±3 599.3) and(6 290.3±3 155.5) ng/mg creatinine, the cotinine contents were(1 047.0±625.4) and(758.9±603.5)ng/mg creatinine. The contents of total nicotine metabolites in the urine of two vaping groups were (5 723.1±3 197.8)and(4 381.6±3 271.3) ng/mg creatinine, and the cotinine contents were(918.0±688.5)and (701.4±670.6) ng/mg creatinine(P < 0.05). When switched to e-cigarettes, the puffing volume was increased, puffing interval shortened, and puffing duration prolonged or unchanged. However, the contents of total nicotine metabolites in the smokers' urine decreased after switching to e-cigarettes based on this small-scale volunteer study.