Abstract: In order to examine the internal quality consistency and acceptability on the grade quality of flue-cured tobacco leaf bales handed over between industry and commerce, C3F samples were used as research materials in this study. Through leaf-by-leaf grading and reduction according to the mixed grade ratios of the original tobacco bale, routine chemical components, aroma substances, polyphenols (rutin and scopoletin) and the differences and consistencies of sensory quality of the samples were analyzed according to the adjacent grade relation table. The results showed that the aroma components' contents, sensory quality score and chemical component usability index of flue-cured tobacco were slightly affected when C3F bales mixed with leaves of adjacent grades in the same group or with similar appearance quality. The overall quality of flue-cured tobacco was greatly affected when the bales were mixed with non-adjacent grades. The content of aroma substances increased and the usability index of chemical components was better when B2F was mixed in the bales. However, the overall quality of the bales was seriously affected by the mixing of tobacco leaves of secondary groups in the original grade.