Abstract: To eliminate the subjectivity and uncertainty of color evaluation for tobacco flavors, a colorimeter was used to measure the colors of different types of tobacco flavors at different dilution ratios and different batches of the same flavor. The results showed that:1) Colorimetry was suitable for the color detection of tobacco flavors, however complex samples with sticky dark color could be only detected after diluting. 2) Colorimetry could accurately presented the sample colors of different flavor types at different dilution ratios and different batches of the same type. 3) Colorimetry featured an acceptable detection accuracy with the relative standard deviations of parallel tests of color indices (L^*, a^*, b^*) were all less than 5%. 4) Colorimetry could directly reflect the color difference between the samples of similar colors which could not be distinguished by visual method. 5) Compared with refractive index and relative density, color difference value was easier to reflect the quality fluctuation caused by the change of dilution ratio.